After removing the spent medium, cells were incubated in 500 l complete MEM containing antibiotics (10 g ml 1 Rif, 50 g ml 1 Mox or 20 g ml 1 Van) with or without 0100 M KL1 or its analogues (KL27), 20 M BHA (Sigma) or 010 M BIX-01294, in the presence of 50100 g ml 1 Gen at 37 C for 624 h
The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters
114 Ni II , like Zn II and Mn II , can perform Lewis acid chemistry, exemplified by two hydrolytic enzymes, the dinuclear Ni II enzyme urease (Section 5.1.2) and the mononuclear glyoxylase-I, but also functions as a cofactor in [NiFe]-hydrogenase and Ni-superoxide dismutase, among others, where both the Ni I and Ni III oxidation states must be accessible
D., et al., The Enzymes of the Oxidative Phase of the Pentose Phosphate Pathway as Targets of Reactive Species: Consequences for NADPH Production, Biochemical Society Transactions 51, no