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HETEROLOGOUS EXPRESSION OF AsGGT1, AsGGT2, AND AsGGT3 IN YEAST The coding regions of AsGGT1 , AsGGT2 , and AsGGT3 were amplified by PCR using the cloned cDNA fragments described above, KOD plus DNA polymerase (Toyobo), and the following gene-specific primers: AsGGT1-FKpn3A (5- GGTACC AAAATGAACCAAATGGCGCCGGCTTC-3) and AsGGT1-stop-RXh (5- CTCGAG CTATACACAAGCAGGACTTC CATC-3) for AsGGT1

Such oxidations are in direct competition with glutathione reductase and ABC transporters which usually maintain very low GSSG concentrations under physiological conditions 5,7,62,63,64