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Beyotime, Shanghai, China) was used to detect ROS generation in pancreatic tissue sections

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Then, the mixtures were placed onto a microplate spectrophotometer (Bio-Rad) for measurement of the absorbance at 340 nm

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276 Moreover, due to the lack of sufficient clinical evidence demonstrating the efficacy and safety of KD, there still are debates on using KD in several diseases, 276 including diabetes, cardiovascular disease, and Alzheimers disease

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