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PLOS ONE , Nov

The cells were washed 3 times with PBS and then incubated with SA--gal staining solution at 37 C overnight

The decrease in the SFR of tobacco chewers and areca nut chewers in comparison to normal subjects is because of the long term of usage of tobacco and the enhanced epinephrine effect of areca nuts, that is, alteration in the autonomic nervous system by increasing the plasma level of epinephrine and norepinephrine, which results in decreased SFR

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