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Enumeration of brain cysts Brains were homogenized in 5 mL of RPMI 1640 medium in a glass potter, and the number of tissue cysts per brain was determined microscopically by counting 10 samples (10 L each) of each homogenate

These GST enzymes catalyze the binding of synthesized glutathione to electrophilic xenobiotics (foreign chemicals), reactive drug metabolites, and environmental toxins, effectively neutralizing their threat

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However, following ingestion of a large overdose (150 mg/kg or greater) the glucuronide and sulfate conjugation pathways are saturated resulting in a larger fraction of the drug being metabolized via the P-450 pathway