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Further, we analyze the methionine cycle sub-network in the Reed-Nijhout model in order to interpret experimental data in light of model predictions

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The chromatogram was recorded via UV absorption at 210 nm

Materials and methods Primary human hepatocyte isolation Primary human hepatocytes (PHH) were prepared from nontumorous tissues obtained from freshly resected human livers of different donors (PHH Ctrl, n = 4) or explanted livers of WD patients (PHH WD, n = 2) via a two-step perfusion protocol as previously described [8]