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Description

HFD differentially methylates vitamin D metabolism enzyme genes and VDR To identify the gene-specific methylation of CpG island of vitamin D metabolism genes and VDR, we adapted a digestion-based gene-specific PCR methylation assay

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Cytokine analysis Enzyme-linked immunosorbent assay (ELISA) is used to detect the expression levels of cytokine in the serum

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A small fraction, however, is oxidized into a highly toxic chemical, NAPQI (N-acetyl-p-benzoquinone imine)

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