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No HPBA formation was observed with either CYP2A6- or CYP2B6-overexpressing microsomes when nicotine was used as substrate in the present studies, a pattern consistent with that observed in previous studies demonstrating a lack of HPBA formation activity by CYP2A6 with nicotine as the substrate

Neither replaces the consistency of a machine, but they're useful supplements if you prefer manual techniques

Well important of note is that a separate study from 2011 seems to complement the present findings, as it showed that smokers who said they enjoyed smoking very much were 30 per cent less likely to have attempted to quit within six months than smokers who said they enjoyed smoking to a lesser degree