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Cell cycle analyses Cell cycle analysis was carried out using the Click-iT EdU Cell Proliferation Assay Kit (ThermoFisher Scientific) according to the manufacturers instructions

[DOI] [PMC free article] [PubMed] [Google Scholar] 44.Vella L.A., Rowley A.H

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Evaluation of the number of sexual lineage cells The optical dissector method was used to determine the total number of cells including spermatogonia, spermatocytes, round spermatids, long spermatids, Sertoli cells, and Leydig cells in 20 m sections (Fig